Ageing & Cellular Senescence
Mechanisms linking altered cellular state to tissue function during ageing.
Research
Cellular ageing, communication & measurable function.
My research experience spans endothelial ageing and senescence, extracellular-vesicle biology, quantitative functional assays and proteomic analysis.
Research Question
How does a change in cellular state affect communication and biological function?
This question was central to my doctoral work on endothelial ageing and extracellular vesicles and remains relevant to how I interpret functional cell-based experiments.
The sections below separate the research into the actual experimental areas in which I worked, rather than treating them as a single linear pathway.
Endothelial ageingExtracellular vesiclesFunctional assaysProteomics
01
Doctoral Research · Endothelial Biology
Endothelial Ageing & SenescenceHow does ageing alter endothelial-cell phenotype?
During my doctoral research, I studied cellular ageing and senescence using primary human endothelial-cell systems.
The work examined senescence-associated phenotype, cellular stress, cell-cycle state and endothelial activation, and how altered donor-cell state could affect experiments with recipient cells.
ModelPrimary human endothelial cells
Biological FocusAgeing, senescence and endothelial phenotype
Experimental QuestionHow altered cellular state affects downstream biology
Conceptual illustration of altered cellular state. AI-assisted scientific artwork; not experimental data.
02
Doctoral & Translational Research
Extracellular-Vesicle CommunicationHow can extracellular vesicles transmit information between cells?
Extracellular vesicles became a major part of my research through work in translational and cardiovascular settings.
My experience includes EV isolation and preparation, particle and biophysical characterisation, cytometric approaches, EV tracking and recipient-cell experiments designed to test how vesicle-associated communication relates to biological effects.
Doctoral Question
Whether age- and senescence-associated changes in endothelial cells are accompanied by changes in extracellular-vesicle composition or recipient-cell effects.
Conceptual representation of extracellular-vesicle release, transfer and recipient-cell interaction. AI-assisted scientific artwork; not experimental data.
03
Postdoctoral Research · Université Paris Cité
Quantitative Functional AssaysHow can intracellular delivery be tested as function rather than uptake alone?
During my fixed-term postdoctoral appointment in the Lavieu Lab at Université Paris Cité, I worked with quantitative mammalian cell-based systems for evaluating functional cargo delivery.
I optimised and standardised NanoLuc-based functional assays in engineered HeLa donor–recipient systems, with emphasis on reproducible workflows, transfection optimisation, experimental controls, quantitative analysis and structured troubleshooting.
Collaborative work also included evaluating cellular uptake and functional intracellular delivery using modified extracellular-vesicle delivery systems.
Central Focus
Whether intracellular delivery produces a measurable functional biological readout.
Conceptual illustration of vesicle–cell interaction linked to a quantitative readout. AI-assisted scientific artwork; not experimental data.
04
Doctoral Secondment · ISAS Dortmund
Proteomics & Molecular ProfilingWhich molecular changes accompany altered endothelial and EV states?
Proteomic analysis added molecular resolution to my doctoral research on endothelial extracellular vesicles.
During work involving ISAS Dortmund, I combined proteomic sample preparation and quantitative analysis with biological interpretation of extracellular-vesicle protein data.
Sample PreparationS-Trap preparation and EV samples for LC-MS/MS proteomics
Quantitative AnalysisMaxQuant · Perseus · R
Biological InterpretationCytoscape · Reactome · FunRich
Areas I Want to Develop Next
Future research interests.
These are directions I want to build into, not claims of established expertise.
Immunology of Ageing & Disease
Immune-cell responses where ageing, tissue stress and intercellular communication intersect.
Quantitative & Technology-Enabled Biology
Reproducible cell-based systems, molecular profiling, imaging and analytical technologies that improve mechanistic research.